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After an incubation interval, the secondary antibody answer is eliminated and versions that were generally adherent are washed off. The final phase is the generation of colored merchandise in wells with extra antibodies and the supplement the molecule substrate. The goal of an ELISA will be to ascertain in case a protein that is distinct is present in a sample and the way much of they occur within the sample. And lastly substrate is added along with the molecule was made by by the sign -substrate reaction is tested. Czytaj dalej